Dear Andrea, we did a lot of work on hematogones and normal bone marrows quantifying antigens on flow, and correlating findings with morphology. pop1 is also TdT positive while pop 2 is TdT negative. We consider both populations as hemotogones as you will find them both increased in cases like ITP,Gaucher's disease, post vaccination or viral infection. pop 2 is always much higher in percentage than pop 1, morphology of both population is slightly immature with population 2 larger in size. Nahla Farahat Lecturer of clinical pathology Faculty of Medicine Alexandria University- Egypt Andrea Illingworth wrote: > Another question for the flow experts:When looking at normal B- cells > in the bone marrow, one can sometimes differentiate 3 different > populations in the bone marrow, representing the various maturational > stages of B-cell differentiation:Pop#1: CD19+, CD10+, CD34+, no k/l. > CD45 dim, very low FALSPop#2: CD19+, CD10+ (a little weaker than the > first pop.), CD34-, weak CD20 and CD22, no k/l, CD45 ( a little > brighter than pop 1), very low FALSPop#3: CD19+, CD20+ (bright), CD22+ > (bright), polyclonal kappa and lambda, bright CD45+ The third > population obviously consists of mature B-cells and the first 2 > populations are immature B-cell precursors. Which ones are the > hematogones, only the first population or is the second population > (CD10+,CD34-) also called hematogones? What is the definition of > hematogones? Are they always CD34+?We were also wondering why there is > no apparent spectrum betwen population 1 and 2, they seem to "jump" > from being CD34+ to being CD34 negative without the stages in > between. Thank you for any insightAndrea IllingworthDCDS Flow > CytometryBangor, Maine
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:01:05 EST