I would be very surprised to find excellent CD103 staining in SLVL. Have you seen CD103 positivity in SLVL? Maryalice >How about a diagnosis of Splenic Lymphoma with Villous Lymphocytes? >Immuno-type will be much like what you describe. Supporting evidence >will be a IgM paraprotein and clinical behavious is similar to that >of a low grade NHL. Confirmation will be obtained on splenic >histology where it typically is a 'red pulp disease as opposed to a >white pulp disease' as it is in Hairy Cell. > >Te-Chih Liu >National University Hospital >Singapore > >-----Original Message----- >From: Maryalice Stetler-Stevenson [mailto:stetler@box-s.nih.gov] >Sent: Saturday, 3 February 2001 12:00 >To: Cytometry Mailing List >Subject: Interesting case >Importance: High > > We have a very interesting case. An 80 year old gentleman >with a WBC= 47 K/uL and a working diagnosis of CLL sought another >opinion. We found his tumor cells to be negative for CD5 and CD23. >They had Bright CD19, CD20, CD22 and surface kappa. They were >positive for CD103, FMC7 & and CD11c, although the CD11c was not the >bright staining characteristic of hairy cell and CD25 was negative. >Also- cells were negative for CD38 and CD10 but + for CD79 and CD24. >The specimen was a submitted heparinized tube- no fresh smear. We >have found that cells sitting in heparin for awhile have poor >nuclear features after smearing and staining. However, the cells >have copius cytoplasm. Borders may be curled, indistinct, possibly >hairy but maybe not. We feel that this is most consistent with >atypical hairy cell. The diagnosis of CLL was never confirmed by >immuno studies- just a low grade lymphoid leukemia- and I feel the >diagnosis is wrong. The clinician asked if this could be PLL. Has >any one ever seen really good staining with CD103 in PLL? > > > > Maryalice > >Maryalice Stetler-Stevenson > >Director Flow Cytometry Unit > >Laboratory of Pathology, NCI, NIH > > >"Learn the rules so you know how to break them properly." > >The Dalai Lama Maryalice Stetler-Stevenson Director Flow Cytometry Unit Laboratory of Pathology, NCI, NIH "Learn the rules so you know how to break them properly." The Dalai Lama
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