Hi, Has anyone heard of a successful way to stain and sort for an intracellular marker (e.g. Ki-67), followed by isolating intact RNA? Our problem so far has been the permeabilization/fixation step, which appears to result in degraded RNA after cell sorting. I would be happy to provide more detail about what we've tried but wanted to send out this first in case there is someone doing this specific type of work and could relate what works for them and/or suggest references. Regards Don Walker The information contained in this email is intended for the personal and confidential use of the addressee only. It may also be privileged information. If you are not the intended recipient then you are hereby notified that you have received this document in error and that any review, distribution or copying of this document is strictly prohibited. If you have received this communication in error, please notify Celltech Group immediately on: +44 (0)1753 534655, or email 'is@celltech.co.uk' Celltech Group plc 216 Bath Road, Slough, SL1 4EN, Berkshire, UK Registered Office as above. Registered in England No. 2159282
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