Hi Maryalice, The few PLL cases we looked at were CD103 neg. Have you done the cytochemical staining for tartrate resistant acid phosphatase? It is considered one of the characteristic features of HCL and I run it in unclear cases. Splenic lymphoma with villous lymphocytes can also be TRAP+ but it should not express 103. Kind regards Anna At 11:00 2001-02-02 -0500, you wrote: > We have a very interesting case. An 80 year old gentleman with a WBC= 47 >K/uL and a working diagnosis of CLL sought another opinion. We found his >tumor cells to be negative for CD5 and CD23. They had Bright CD19, CD20, >CD22 and surface kappa. They were positive for CD103, FMC7 & and CD11c, >although the CD11c was not the bright staining characteristic of hairy cell >and CD25 was negative. Also- cells were negative for CD38 and CD10 but + >for CD79 and CD24. The specimen was a submitted heparinized tube- no fresh >smear. We have found that cells sitting in heparin for awhile have poor >nuclear features after smearing and staining. However, the cells have >copius cytoplasm. Borders may be curled, indistinct, possibly hairy but >maybe not. We feel that this is most consistent with atypical hairy cell. >The diagnosis of CLL was never confirmed by immuno studies- just a low >grade lymphoid leukemia- and I feel the diagnosis is wrong. The clinician >asked if this could be PLL. Has any one ever seen really good staining with >CD103 in PLL? > > > Maryalice >Maryalice Stetler-Stevenson >Director Flow Cytometry Unit >Laboratory of Pathology, NCI, NIH > >"Learn the rules so you know how to break them properly." >The Dalai Lama Anna Porwit Hematopathology Lab. Department of Pathology, Radiumhemmet Karolinska Hospital, 171 76 Stockholm, Sweden Anja.Porwit@ks.se tel.:+46-851774518 fax.:+46-851775843
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