At our lab we run high speed sorting on a BD Vantage SE to collect CD8+ gamma delta cells from bovine peripheral blood. This cell line represents about 1% of the lymphocyte population. At high speed sorting the purity we get is not optimal( ranging from 55% to 90%). This is mostly dependent on sort speed and staining intensity (seperation of populations). My question is: has anyone done straight-ahead sorting for rare cells. In straight-ahead sorting the sorted droplets have no charge placed on them. The unwanted cells are charged and diverted to the right or left. How does one set up the instrument to collect the rare cells? Is recovery tuely close to 100% and does it help with purity? Thanks for any help, Larissa
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