Re: CD10 expression

From: Anja Porwit (Anja.Porwit@ks.se)
Date: Thu Feb 01 2001 - 05:05:07 EST


Hello,
We use the same CD10 clone from DAKO, SS2/36 PE conjugated.
BTW, the expression of CD10 in germinal centers of reactive follicles has
also been confirmed by immunhistochemical stainings:
McIntosh GG, Lodge AJ, Watson P, Hall AG, Wood K, Anderson JJ, Angus B,
Horne CH, Milton ID. NCL-CD10-270: a new monoclonal antibody recognizing
CD10 in paraffin-embedded tissue. Am J Pathol. 1999 Jan;154(1):77-82.
We use tonsils with hyperplasia as a control for CD10 immunohistochemical
stainings.
Kind regards
Anna


At 10:31 2001-02-01 +0100, you wrote:
>Dear Anna,
>

>
>I would like to ask about the manufacturer and clone of the CD10
>antibody you use. We use DAKO CD10 (SS2/36 clone) but we don't see a
>significant population of CD10 positive B cells in cases of reactive
>follicular hyperplasia, only in follicular lymphoma.
>Thanks.
>
>Best regards,
>
>
>
>
>
>--
>Pal Jakso
>
>University of Pecs
>Faculty of Medicine
>Dept. of Pathology
>12. Szigeti str.
>7643, Pecs
>Hungary
>email: jakso@apacs.pote.hu
>
Anna Porwit
Hematopathology Lab.
Department of Pathology, Radiumhemmet
Karolinska Hospital, 171 76 Stockholm, Sweden
Anja.Porwit@ks.se
tel.:+46-851774518
fax.:+46-851775843



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