Dear Lynn, Beside tetramers, there are other new approaches to detect antigen specific T cells based on cytokine production (i.e. cytokine release assay from Miltenyi Biotec) which works also with a frequency = or <0,1% positive cells. If you can achieve a very good negative control with low background (<0,01%) then the assay is pretty reliable. Hope it helps, Corrado '''''''' -------------------ooo--O..O--ooo-------------------------- Corrado M. Cilio, M.D., Ph.D. Endocrine Research Unit Wallenberg laboratory Malmo University Hospital Lund University S-205 02 Malmo, Sweden Tel: +46-40-332395; mobil: +46-70-4330338; Fax: +46-40-337042 e-mail: corrado.cilio@cmb.umu.se -------------------------------------------------------------- Judge a man by his questions rather than his answers. - Voltaire -
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