Brian On the MoFlo you can set up any pattern your heart desires. A "monolayer"??? don't know about that unless maybe put onto poly-L-lysine. Larry At 01:58 PM 1/26/2001 -0800, you wrote: >Dear Dr. Arnold, > >I saw your email reply concerning sorting onto glass slides and I wanted to >ask you a question about this. Can you sort in a pattern, where cells are >distanced from one another, or can you at least put down a monolayer? Any >info. at all would be helpful to me. Thanks very much-- > >Brian > > > > > >Works fine. Cells will be a bit dilute. I have sorted with my MoFlo > >onto a slide put on a cover slip and gone straight to my LSC. Cells are > >fine. Usually put about 1000 cells down for a 22mm2 cover slip suing the > >150um tip so could do more with a 70 or 100um tip. > > > >Larry > > > >At 03:09 PM 1/22/2001 -0500, you wrote: > > > >>One of my colleagues here was asking me if it was possible to high speed > >>sort onto a glass slide. I've sorted into microtitre plates but never > tried > >>slides....anyone tried this? > >> > >>if so how bad was the charge build up from the stream? > >>were the cells still viable or do they go SPLAT? > >> > >>Cj Jett > >>Scientist: Biomedical Development > >>Compucyte Corp. > >>(617)-577-3811 > >>12 Emily St. > >>Cambridge Ma 02139-4507 > > > >Larry W. Arnold, Ph.D. > >Res. Assoc. Prof. > >Director, Flow Cytometry Facility > >Department of Microbiology and Immunology > >Lineberger Comprehensive Cancer Center > >CB# 7290 > >University of North Carolina at Chapel Hill > >Chapel Hill, NC 27599 > >Phone: 919-966-1530 > >FAX: 919-962-8103 > > >Brian Windsor, Ph.D. >Texagen, Inc. >Lloyd Lab MBB 1.448 >The University of Texas at Austin >2500 Speedway >Austin, Texas 78712 >512.471.3553 >Fax: 512.232.3432 >b.windsor@mail.utexas.edu Larry W. Arnold, Ph.D. Res. Assoc. Prof. Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103
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