G1 and G2 determination

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Hello fellow flowers,

     WE are atttmepting to determine G1 and G2 cycles of a tumour cell
line in response to various treatments.  WE have used a PI stain and
elimintated doublets.  What we were wondering is if there is an accepted
protocol for analysis of the G1 and G2 peaks.  Particularily, the G2 peak,
which is evident in some of our samples, but not others.  In the samples
with no evident G2 peak, there is still some baseline fluorescence or
'noise', but no disernable peak.

What is the acceptable practice for determination of the G2 peak, and
method of analysis when there is no disernable G2 peak?

Any input is most welcome.

Thank you for your time and valuable advice,
Janet

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