Our cytometer is up and running again, with many thanks to everyone who emailed suggestions and in particular Steve Merlin. It turns out that one of the lines under a pinch valve was occluded with crystals. Once this was cleaned (as well as a compounding error created by yours truely) I was able to run beads through the instrument. I also went to the research center that donated the instrument and was able to find additional documentation for it. Of course, in the manual were directions for what to do every two to four weeks to avoid crystal formation in the lines! Again, thanks to the many of you who helped me resolve this problem. Regards, Tony -- Tony Schountz, Ph.D. Department of Biological Sciences Mesa State College mailto:tschount@mesastate.edu
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