Hi folks, I have a user who is just getting started w/ apoptosis in a fibrosarcoma cell line (HT1080). Does anyone have a best guess for a reliable positive control treatment (camptothecin, UV, etc?). All our tests have been negative to date, but we can't say it's not occurring unless we verify our assay. (We're using PI at this point, although I'm trying to talk them into "graduating" to tunel or annexinV.) Thanks in advance, Steve +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Steve G. Hilliard flowman@uga.edu Flow Cytometry Facility 542-9474 University of Georgia
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