At 3:36 PM -0500 11/1/01, Mario Roederer wrote:
>
>Unfortunately, CellQuest was never written with the generation of
>publication quality figures in mind. Hence, all of its displays (as
>far as I can tell) are "bitmap" representations, with no vector/font
>information. Hence, it is difficult to change things like colors,
>fonts, etc.--you must use a program that can do bitmap editting and
>then overlay with your own text/line information. (Witness the
>contortions people have to go through to make publication-quality
>graphics!)
The new version of CellQuest ("CellQuest Pro") has supposedly
addressed some of these issues (according to the Australian rep I
spoke to at a recent conference) but I haven't had a chance to see it
in action yet (other than the "movie" on the BD website...)
> FlowJo was indeed designed to do publication quality output
....
> When you copy from FlowJo and paste into any graphics application, you can
>then ungroup the elements, select individual lines (or contours, dots
>in dot plots, etc.), text items, or whatever, and manipulate them at
>will.
>
>For some examples, see
><http://www.treestar.com/flowjo/v3/html/pubgallery.html>. For other
>examples, see any of my publications... in particular, I'd like to
>take this opportunity to advertise the upcoming February issue of
>Nature Medicine, in which we have a New Technology article about
>11-color flow cytometry!
>
FlowJo really does excel in this area. I can always pick who is using
FlowJo by the quality of flow figures in their papers (eg recent
papers by McHeyzer-Williams et al show FlowJo plots to good effect,
and we had a paper in the Dec. issue of Immunity with (simple) FlowJo
plots).
Adrian Smith
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