Medimachine for functional studies

From: Dan on Wesley (holterda@wesley.evms.edu)
Date: Thu Jan 18 2001 - 09:58:04 EST 

Hello fellow flowers;
    We are a Tumor Immunology Lab and I wonder if anyone has used the B-D
medimachine to dissociate mouse tumors.  We currently digest with enzymes
for phenotyping but have recently been concerned that the functions of the
infiltrating immune cells may not reflect their true characteristics.   We
would like to correlate phenotypic changes with functional changes in these
cells.  I would appreciate any advice .
   Sincerely,

   Daniel Holterman
   Eastern Virginia Medical School

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