Hi Marcos, I use 10% human AB serum to incubate the cells for 20 minutes at
4 degrees, then wash and pellet the cells. Afterward you can use your
antibodies. I hope it will help. Regards Rafael
>Hi colleagues,
>
>I tried to browse the archive to find the answer to this question, which I
>think might have been addressed before, but without success.
>Does anyone have a protocol for fc-receptor blocking with human IgG when
>staining ficoll-separated PBMCs? I usually work with 0.5 to 1 x 10 to the 6
>cells per tube. My routine conjugates are CD3-APC, CD4Pe or FITC and
>CD8Cy-chrome.
>Thank you,
>
>Marcos da Silva
>Pediatric Immunology lab - UMass Medical School
>Marcos.DaSilva@umassmed.edu
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(o o)
________________________________oOo__(_)__oOo_________________________________
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/ o \/| | University Inst.for Virology http://www.vetvir.unizh.ch/
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