Bill, have a look at these: Bertoncello I, Hodgson GS, Bradley TR. Related Articles Multiparameter analysis of transplantable hemopoietic stem cells: I. The separation and enrichment of stem cells homing to marrow and spleen on the basis of rhodamine-123 fluorescence. Exp Hematol. 1985 Nov;13(10):999-1006. PMID: 2865163; UI: 86030571 Wolf NS, Kone A, Priestley GV, Bartelmez SH. Related Articles In vivo and in vitro characterization of long-term repopulating primitive hematopoietic cells isolated by sequential Hoechst 33342-rhodamine 123 FACS selection. Exp Hematol. 1993 May;21(5):614-22. PMID: 8513861; UI: 93292604 Leemhuis T, Yoder MC, Grigsby S, Aguero B, Eder P, Srour EF. Related Articles Isolation of primitive human bone marrow hematopoietic progenitor cells using Hoechst 33342 and Rhodamine 123. Exp Hematol. 1996 Aug;24(10):1215-24. PMID: 8765497; UI: 96348007 Carl-Magnus Hogerkorp Dept. of Immunotechnology University of Lund Sweden -----Original Message----- From: Bill Telford [mailto:telfordw@box-t.nih.gov] Sent: Wednesday, January 03, 2001 4:16 PM To: cyto-inbox Subject: Side population detection Hello everyone... We have a group at NIH doing side population analysis using Hoechst 33342 by the standard technique. My (probably obvious) question is...is there something special about the Hoechst dye (mdr type specificity, nuclear localization, etc) that makes it the only mdr susbtrate effective for stem cell identification? Is it possible to use other fluorescent mdr substrates (i.e. rhodamine 123, daunomycin, etc.) to identify these cells? Thanks in advance! Bill Telford DCS-NCI-NIH
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