Patient Sample sorting for FISH.

From: Chris Worth (caw@bcc.louisville.edu)
Date: Wed Jan 03 2001 - 12:10:50 EST


Hey gang,

The Clinical folks here want to sort cells to use for FISH.  Apparently, we can't fix the
cells.  What are people's thoughts on this?  I've spent all of my time sorting
Transfectants, and fixed cells.  So biohazardous samples are a new thing for me.

I've got an Elite, and  a MoFlo I can sort with.

I'm not overly excited about making millions of little droplets with unfixed patient
material in them.

So

1) can this be done safely, and how?  Tips etc. for each instrument will be greatly
appreciated.

2) is there a FISH procedure we don't know about that would work with fixed cells?
Which would negate the problem of unfixed material.


Thanks,

Chris



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