Dear Flower's, Wish you all a Merry Christmas and a Happy and Prosperous New Year. I know everybody is in a holiday mood but I am finding some thing peculiar and wanted your advice... One of my collegue is looking at GFP expression in a cell line. He prepares the sample and we acquire the cells immediately on a FACS Vantage SE and have found good results. However if due to any reason he has to keep the cells at 4oC usually for 2-4 hrs and then we acquire the cells, the cells seem to loose their FL1 signal. This has been done twice and both the times we have got the same results. Any ideas as to what is going on. Does GFP show some changes based on temperatur. This vector was obtained from Clontech. Thanks for your replies in advance. Regards Rana ____________________________________________________________________ Get free email and a permanent address at http://www.netaddress.com/?N=1
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:41 EST