Candida Facs

From: Idit Hazan (ihazan@uci.edu)
Date: Fri Dec 22 2000 - 12:57:11 EST

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I have a question about FACS for DNA content in Candida albicans. Is it
possible that a candida hyphal cell, which consists of a long string of
cells (in my case 2 cells attached) separated by septum, with individual
nuclei in each compartment are counted by the detectors as 2 cells with 2n
content each rather than one cell with 4n content. the nuclei are about 20-30
micron apart, but, as I mentioned, are attached on one long hypha.
Idit

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