On a BD Calibur or FACScan, the endreduplicated cells can usually be clearly distinguished on a dotplot of width versus DNA area. On the Beckman Coulter XL or Elite, a plot of DNA peak versus DNA area will show these cells. It is often easier on these machines because the laser beam has a narrower width. I have attached a couple of examples taken from one of the lectures on my Internet course. The region encompasssing single cells ahs nee extended to include a small percentage of endoreduplicated cells. I have seen endoreduplicated cells quite commonly in cell lines. It can be quite a problem as often the properties of the cells change and the worker does not realise that his/her long term cultures are changing. I always advise people to check the ploidy of long term cultures regularly; but they seldom take my advice! Michael Ormerod 34 Wray Park Road Reigate RH2 ODE Telephone: +44 (0)1737 241726 FAX: +44 (0)1737 226736 Mobile telephone: 07802 293242 Web site: http://ourworld.compuserve.com/homepages/Michael_Ormerod
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