We have had excellent results with the Alexa 350 dye + instructions and 488 labeling kit. We have not tried the 568. The procedure is extremely easy and we obtained consistent results from batch to batch. We have used FITC kits in the past and the labeling procedure is similar in degree of difficulty. George Babcock University of Cincinnati School of Medicine Cincinnati, OH At 01:22 PM 12/6/00 -0600, Lynn Dustin wrote: >Hello all, >A colleague wants your honest opinions of the Alexa dye conjugation kits > >(particularly Alexa Fluor _488_ and _568_) from Molecular Probes. >Reliability, ease of use, alternatives to consider. His application is >direct dye conjugation to monoclonal antibodies for fluorescence >microscopy. >Thanks in advance for your opinions and your help! >************************************** >Lynn Dustin, Ph.D. >Assistant Professor >Molecular Microbiology and Immunology >St. Louis University School of Medicine >1402 S. Grand Blvd. >St. Louis, MO 63104 >Phone: 314-577-8415 >Fax: 314-773-3403 >Email: dustinlb@slu.edu >
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