Dear Flowers, Due to some errors in the mail adress I am not sure if my last querry reached you...So I am sending the problem again. We try to use green-fluorescent BODIPY conjugated hyaluronic acid (Molecular Probes) to study binding of hyaluronian by human monocytes. The problem we can't resolved is very high (>90%) dose undependent binding of this compound to the surface of monocytes. Morever, this binding is not blocked by the use of unlabelled hyaluronic acid, so it seems to be completely unspecific. Does anyone have an experience with the use of this compound? Is it possible that human monocytes preferentialy bind BODIPY? Any comments will be appreciated. Best regards, Jarek Baran, Ph.D. Department of Immunology Polish-American Institute of Paediatrics Cracow, Poland
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