Cindy Levine wrote: >I'm trying to estimate the % of "simple" single parameter flow cytometry >studies done both with analytical and sorting flow cytometers. By single >parameter I'm referring to cell cycle, apoptosis, GFP+/- assays. Could >anyone give me the approximate percentages in their facility? Is this market research? I suppose it doesn't matter, but the way you have phrased the question won't get you the answer you may need. These aren't quite "simple" assays, and they aren't quite "single parameter" assays. Cell cycle is probably closest to "simple single parameter", in that it can be done with a flow cytometer measuring only a single (fluorescence) parameter. Apoptosis and GFP+/- assays typically require at least two parameters (one scatter, one fluorescence) for triggering and gating (and a second scatter parameter may be helpful there), meaning a more complicated instrument. So, if you are trying to find out whether a lot of labs could use a simple instrument that only measures a single fluorescence parameter, and you assume they can because they people tell you they do a lot of "simple single parameter" assays, you'll get the wrong answer. What you want to ask in that case is how many assays they do which measure only a single optical parameter and rely on only that parameter for triggering and gating, or get more specific and ask about the percentages which use scatter only, fluorescence only, one scatter and one fluorescence, and multiple scatter and fluorescence parameters. But I'm only guessing why you want the information. -Howard
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