Frank, AO noise is usually the opposite . . . signal increases over time. Also, brief bleach treatment will clear that quickly. Snezna, I noticed later in your message that you do NOT see the effect in light scatter . . . so, this leads me to think electronics. MAK. FACS_COPY@wehi.edu.au wrote: > Snezna, > > You haven't had someone running acridine orange have you? Sounds like > the fluorescence builds in the line and is flushed out by the sample. > > Frank. > > Frank Battye \ / < The Cytometry Laboratory > Dora Kaminaris \__/ <<<<< The Walter & Eliza Hall Institute > Viki Lapatis ------!!<<<<<<<< Victoria 3050, AUSTRALIA > Jennie Chan /!!\ <<<<< ph: 61_3_9345 2540 fax: 61_3_9347 0852 > Catherine Tarlinton o !! \ < in:: "facs_copy@wehi.edu.au" > Geza Paukovics -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry <http://www.cancer.med.umich.edu/flow_cytometry> phone: 734-647-3216 fax: 734-936-7376 kukuru@umich.edu
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