>From: Kent Claypool <kclaypool@sprd1.mdacc.tmc.edu> >To: "'Kerstin'" <beckk@sun11.ukl.uni-freiburg.de> >Subject: RE: specific DC-Marker >Date: Sat, 30 Sep 2000 10:43:04 -0500 >Importance: high >X-Priority: 1 (Highest) >Organization: UT MD Anderson Science Park Research Division >MIME-Version: 1.0 >Status: > >Hi Kerstin > >One of our PI's is using CD83 and CD86 to resolve immature from mature in >dendritic cells from Rhesus monkey lymphocytes. Also uses HLA-DR, for >example HLA-DR-FITC vs CD83-PE & HLA-DR-FITC vs CD86-PE. Not sure but I >think the expression of CD83 goes up after maturation and CD86 goes up >significantly upon maturation. To learn more this web site is PROW (Protein >Reviews on the Web) http://www.ncbi.nlm.nih.gov/prow/ >From the PROW web site which has much more info including references >MAIN CELLULAR EXPRESSION OF CD83 >* Non-follicular dendritic cells >* Circulating dendritic cells >* Interdigitating dendritic cells within lymphoid tissues >* Langerhan's cells >* In vitro generated dendritic cells >* Thymic dendritic cells >* Weak reactivity with some lymphoblastoid cell lines and with some >germinal center B cells in vivo > >MAIN CELLULAR EXPRESSION OF CD86 >* CD86 is expressed constitutively by interdigitating dendritic cells in T >zones of secondary lymphoid organs and at lower levels by Langerhans cells >and peripheral blood dendritic cells >* It is expressed by memory B cells and germinal center B cells; >centrocytes express more CD86 than centroblasts. Small sIgD+,IgM+ tonsil B >cells do not express CD86 but can be induced to express high levels of this >molecule on activation in vitro through their surface immunoglobulin, CD40, >MHC class II molecules or by phorbol myristate acetate with ionomycin. >These signals also up-regulate the expression of CD86 on memory B cells and >GC B cells >* CD86 has also been reported to be expressed at low levels by monocytes >and this expression is increased by culture with g-interferon >* Endothelial cells (Seino et al. 1995), and T cells activated by CD3 >ligation have also been reported to express CD86 (Das et al. 1995); >* Many T cell clones express both CD86 and CD80 > >Hope this helps. Best wishes! > >Kent > >http://sciencepark.mdanderson.org/flow/ > > > > > >-----Original Message----- >From: Kerstin [SMTP:beckk@sun11.ukl.uni-freiburg.de] >Sent: Thursday, September 28, 2000 11:42 AM >To: Cytometry Mailing List >Subject: specific DC-Marker > > >Hello everybody, >does anyone know a specific marker (except CD1a) for Dendritic cells which >express mature and immature DCs? I want to seperate Monocytes, makrophages >and DC with FACS. Are there any differences in expression between >dendritic cells generated of CD34+progenitor cells, DC generated of >Monocytes or DC of the skin? >Thanks for your help. >Kerstin > >***************************************** >Kerstin Beck >Institut fur Medizinische Mikrobiologie und Hygiene >Abteilung Virologie >Hermann-Herder-Strasse 11 >D-79104 Freiburg >Telefon : +49-(0)761-203-6646 >Fax : +49-(0)761-203-6603 >beckk@ukl.uni-freiburg.de >***************************************** > ***************************************** Kerstin Beck Institut für Medizinische Mikrobiologie und Hygiene Abteilung Virologie Hermann-Herder-Strasse 11 D-79104 Freiburg Telefon : +49-(0)761-203-6646 Fax : +49-(0)761-203-6603 beckk@ukl.uni-freiburg.de *****************************************
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