Re: BD LSR
From: Bill Telford (telfordw@box-t.nih.gov)
Date: Tue Oct 03 2000 - 17:24:21 EST
Hi LuAnn...
We demo'd the LSR at the NIH last spring and ran indo-1 on it, comparing
it with our Vantage SE with a krypton laser emitting in the UV at 100
mW. Both instruments showed comparable sensitivity as reflected in
the bound/unbound indo-1 ratio, although the FACSVantage was slightly
more sensitive in detecting the longer wavelength unbound indo-1 signal
(probably a consequence of somewhat poorer excitation of this form by the
HeCad 325 nm laser line). The LSR uses narrow bandpass filters
similar to those on the Vantage (405 and 485 nm) and a 470 LP dichroic to
split the signals. Full information about our brief experience with
the LSR is at our web site -
http://www-dcs.nci.nih.gov/branches/medicine/flowcore/lsr.htm.
The LSR was fairly simple to use once it was aligned
correctly. Laser and optical alignment are fixed as in the FACScan
and FACSCalibur. Alignment is a less than trivial exercise best
handled by a field engineer.
Bill Telford
At 12:14 PM 9/29/00 -0400, luann wrote:
>
>
>
>Hi Flow-ers,
>
>Our flow lab is in the fortunate position of being able to add
another
>analyzer to our core facility. One of our researchers would
like to add uv
>capabilities to the lab, specifically for Ca++ measurements. At
present we
>are trying to determine the best method to to accomplish this.
The lab
>currently has an Elite sorter with gated amplifier that has an Argon
and a
>HeNe laser, and an XL analyzer. We are currently evaluating
whether it is
>preferable to buy a HeCd for the Elite and then buying a separate
analyzer
>( XL or FacsCalibur), or wherther we should purchase the BD-LSR (with
three
>lasers). Since this is a new machine, I'd appreciate any
comments from
>those who have this instrument, as well as comments from others who
have
>faced a similar choice. I am most concerned with issues
regarding
>reliability, easy of use, and utility of the LSR.
>
>Comments may be sent directly to me at:
>
>luann+@pitt.edu
>
>Thanks all,
>
>LuAnn
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: Sat Mar 10 2001 - 19:31:38 EST