At 08:17 PM 10/02/2000 -0400, Liza McGuire wrote: >Is there any possibility to freeze bags and vials in a rate control freezer >simultaneously? There are many issues why this would not work, but I >wonder if anyone ever tried it and can provide any input. Our standard method for cryopreserving all our clinical products involves freezing bags and vials together in a programmable freezer. The product bag is held in a metal press, with the temperature probe placed next to the bag. A rack containing sample vials from the product rests on the metal press, and the vials and product bag are frozen together. Cell recovery from the cryovials is not quite as high as from the product bag, but still very acceptable. You may want to look at Jershin, et al., Hematopoietic progenitor cells frozen in ampules are satisfactory reference vials for bulk freezing. Progress in Clinical & Biological Research. 389:657-61, 1994. Best of luck, Scott -------------------------------------------------- Scott R. Burger, M.D. Director, Cell Therapy Clinical Laboratory University of Minnesota, Fairview-University Medical Center 420 Delaware Street S.E., Mayo Mail Code 609 Minneapolis, MN 55455 612-626-4919 612-625-2199 - Fax burge009@tc.umn.edu http://www.mbbnet.umn.edu/labs/ctcl.html
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