> Has anyone done any work with intracellular cytokine staining for Flow Cytometry with feline white blood cells? I have a client who wants to measure MAPk using flow. We need to get into the cells and have tried using a home made permeabilization buffer used in intracelluar staining. We are not having great results and have learned over the years that any new species is a whole new ball game, so we are starting at step one. > If anyone out there is doing feline intracelluar staining would you be kind enough to let me know what permeabilzation buffer you are using. Is it a a commercially prepared buffer, which one and how much do you use. Is it a home made buffer, would you be kind enough to send the recipe and quantities used in relation to cell number. > Thanks in advance to all with advice. > Joan K Flow Cytometry Lab Supervisor VMRCVM (540) 231-4115 FAX 540-231-7367 jkalnits@vt.edu "It is better to serve than to receive." B. Borg
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:38 EST