Bill-- For human placenta, check out Ritson and Bulmer, J Immun Methods, 1987, 104:231-6. Be aware, however, that enzymatic dispersal methods do tend to affect cell phenotypes. If you are interested in looking specifically at human intervillous blood, check out the following refs: 1) Rasheed et al., J Immunol Methods, 1992. 146:185-93. 2) Camelo et al., Fetal Diagn Ther, 1995. 10:322-325. 3) Moore et al, J Immunol Methods, 1997. 209, 93-104. We use the latter routinely. It's very gentle and yields a huge amount of maternal intervillous blood cells that are free from fetal cell contamination. Good luck! Julie Moore Date: Wed, 27 Sep 2000 13:49:27 -0700 To: cyto-inbox From: Bill Hyun <hyun@cc.ucsf.edu> Subject: placental digestion Hello, Does anyone have a protocol or reference for digesting mouse or human placenta to single cells for sorting? Thanks. -Bill -- ***************************** William Hyun, Director Laboratory for Cell Analysis UCSF Cancer Center, Room S129 San Francisco, CA 94143-0808 Office (415)476-2632 Lab (415)476-2631 FAX (415)476-8218 email hyun@cc.ucsf.edu Deliveries: 2340 Sutter Street, Room S129 San Francisco, CA 94115 Julie Moore, PhD Center for Tropical and Emerging Global Diseases AND Department of Medical Microbiology and Parasitology College of Veterinary Medicine University of Georgia office: 706-542-5789 lab: 706-542-6758 fax: 706-542-0059 email: julmoore@calc.vet.uga.edu
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