I'm stumped: I activated whole blood with LPS for 4hrs, in presence of BFA, with all of the pertinent controls. I ran the assay 3 times, it only worked once. By "worked" I mean I detected IL-10. Is there some other way to get a "high" control for IL-10? Any human Leukocyte will do. I am using a Rat-anti-human IL-10, IgG2a, R-PE conjugated. Also, what would be the range of cells positive for IL-10 in this setup? I'm using the BD protocol found at http://www.bdfacs.com/literature/brochures/cytokine.pdf any help would be appreciated, Maciej PS. other cytokines run in parallel worked - IL-1b and TNFalpha. So it's not the permeablization step as far as I can tell, nor any of the wash steps or experimental setup. ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Yahoo! Calendar - Get organized for the holidays! http://calendar.yahoo.com/
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:38 EST