TLC and DLC using flow

From: Rana Nagarkatti (rana_nagarkatti@usa.net)
Date: Tue Nov 14 2000 - 16:17:03 EST


Dear Flow'ers,
 Hello,
 Does any one have a paper or reference which can guide me about doing a
Differential leukocyte count and a total LC on a flow cytometer. We have a
three colour flow cytometer with 488nm laser. We are planning to do these
counts from peripheral blood preserved in ACD. Any suggestions would be
helpful. Can I buy the antibodies and do it reasonably accurately without
buying kits as it would be very expensive for routine use.
Thanks,
Regards
Rana.

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