David, if you look at our paper (Rabin et al J Immunol April 1999) you will see that there is no calcium flux by naive T cells isolated from adults. I am sure this is what you are seeing with the cord blood. Short term activation with antiCD3 (3 days) will confer upon them a calcium response to SDF-1. Ronald L. Rabin, M.D. Research Fellow Cytokine Biology Unit Laboratory of Clinical Investigation NIAID/NIH Building 10/Room 11N228 10 Center Drive MSC 1888 Bethesda, MD 20892-1888 Phone: 301-402-4910 FAX: 301-402-0627 > ---------- > From: David Dombkowski > Sent: Tuesday, November 7, 2000 6:23 AM > To: Cytometry Mailing List > Subject: Calcium Flux on T cells in Cord Blood > > > We have run calcium flux on isolated T cells and CD34 cells with SDF as > stimulus. There is a resonable response to SDF by CD34 cells but not by T > cells either CD4 or CD8. I am interested in any comments as to the > non-response of T cells to SDF. We have repeated experiment 2 times and > have loaded cells with Indo-1. There is no indication that loading of T > cells with Indo-1 is a problem as they respond to ionomycin as a positive > control. Thanks. > > David M. Dombkowski > dombkowski@helix.mgh.harvard.edu > Flow Cytometry-Pathology-CNY rm7017 > 149 13th Street > Massachusetts General Hospital-East > Boston, MA 02129 > Tel. (617)726-1683 > Fax.(617)724-3164 > > > >
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