I am looking for some top tips about flowing normal human marrow. We are interested in looking at markers such as CD3,CD20 and CD14. What is the best way to store these samples i.e. for 24h and does anyone 'prepare' the cells before flow i.e. lymphoprep. I have flowed leukemic marrow in the distant past, but I am sure things have changed in the last few years, so any advice is most welcome. Many thanks, Andy.
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