I have a researcher who is new to flow but is very keen to pursue the isolation of CD11c DCs from murine peyer patches. Can anyone send me details of an easy to follow protocol or reference that I can pass onto him, that will allow him to clean up the sample he will eventually bring me to sort four ways. I don't want to waste both his money and my time trying to sort a sample that will simply clog up the hardware every few minutes. Thanks Wayne Turnbull Flow Cytometry Servcies Immunobiology Guys Hospital London
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