Hi all Not strictly a flow question, but I plan on using flow to examine the cellular response I wanted to look at the human immune response to particular antigens. Iwanted to add antgien to human leukocytes in culture and detect the immune response by flow and proliferation assays. I realise however that the immune response will involve a very small number of T cells specific for the antigens, so I probably wouldnt be able to detect the response. Though I would look by flow for the activated T cells and examine them only. Any hints, comments or suggestions on whether it will work or how I can get it to work. Any references you know of that may be useful Thanks Heather Medbury Sydney Aus.
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