Hello all, As Mario Roederer reminded me, the second half of my suggestion was wrong. I agree that TCR+ cells are always CD3+ and what I was talking about were the CD3+CD4-CD8- (quite an opposite situation than what was observed in the mouse tissue). Sorry for my confusion. Ondrej >> Hello, I am pretty much a FACS beginner. I have a question, which >>nobody has been able to answer so far. Every time I do a FACS scan from >>peribronchial lymph nodes (naive BALB/c mice) and stain it for cd3, cd4 >>and cd8 cells I see a huge population of cd4pos/cd3neg cells, as well as >>cd8pos/cd3neg cells. Does anybody know what types of cells these are? I >>have to admit that isolating PBLN >from naive mice is rather tricky, >>usually leaving me with extracting the entire hilus and meshing that. >>Could these cells derive from Thymus (which I am pretty sure to extract >>beforehand) or from oesophagus? Thank you very much, Ph. Stock > >In adidtion to the Hai Qi's comment, CD4+CD3- are also >monocytes/macrophages and CD8+CD3- are also some NK cells. >Both of them could be TCRgamma/delta+ or "true double negatives" (I mean >CD4-CD8-TCRalpha/beta+). I don't know what percentages you should expect in >your mouse tissue, though. >Good luck. >Ondrej > Ondrej Hrusak, M.D., Ph.D. Institute of Immunology Ondrej.Hrusak@lfmotol.cuni.cz fax +4202 2443 5962 http://www.lf2.cuni.cz/clip/ phone +4202 2443 5969, +4202 2443 2084 postal address: V uvalu 84 150 06 Praha 5 Czech Republic
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