Dear Flow landers, I am trying to shave the receptors off the cells surface using trypsin. After this process i like to stain them with conjucated Ab for a particular receptor. Flow is not able to pick up the residual receptors. I like to look at them in a scope after staining with PE/FITC Ab. Any protocol for this ? Any idea and suggestions! Thank you for your time and help in advance. Thank you Mathi Meyer Pharmaceuticals Irvinve ca 92614 949 833 0711.
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