Dear Maryalice, I agree with you completely. When something is unusual one always has to exclude the artifact. I did not notice what you describe about CD19/CD5. Can that depend on antibodies/conjugates you use? Kind regards Anna At 10:02 2000-10-18 -0400, you wrote: >I agree that occasional CLL's can be CD8+, as well as other T-cell >antigens. If you do enough cases you run into all kinds of weird >things and if you do tons you will get a series of CD8+ or CD2+ or >CD34+ or what ever. I think, though, when ever you encounter >something so unusual, you should first make sure that it is real. The >T-cells in unwashed blood from some normal individuals will >apparently stain positive for CD4 and CD8. I can send you some as my >blood does this. This is disturbing to say the least. However, it is >an artifact. This points out that artifact can occur, even when you >have great personnel with excellent technique. I always investigate >unusual findings before I accept them. Then, when you put together >your series and publish you (and everyone else) are sure it is real. >You may find that some cases are real and some are not. By thw way- >all my B-cells also appear to coexpress CD19 and CD5. When you wash >the blood this goes away, along with an excellent case report of an >apparently normal (although some may question this) individual with >all T cells coexpressing CD4 and CD8 and all B cells coexpressing >CD19 and CD5 but polyclonal. > > Maryalice > > > >>I think that the occasional positivity for CD8 in CLL is real - we have >>come across a couple more of these cases since the first one in 1987. It >>has been checked after washing and with various CD8 conjugates. These cases >>do not differ clinically from "regular" CLL. >>Kind regards >>Anna >>Anna Porwit >>Hematopathology Lab. >>Department of Pathology >>Karolinska Hospital, Stockholm >>Anja.Porwit@ks.se >>tel.:+46-851774518 >>fax.:+46-851775843 > >Maryalice Stetler-Stevenson >Director Flow Cytometry Unit >Laboratory of Pathology, NCI, NIH > Anna Porwit Hematopathology Lab. Department of Pathology Karolinska Hospital, Stockholm Anja.Porwit@ks.se tel.:+46-851774518 fax.:+46-851775843
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