Hi Deneen,
We routinely run both research and clinical specimens on the same
instrument. Cleaning is not an issue unless a dye such as PI is used(sticks
to the tubing). Then we clean the sampler with bleach or 2% Contrad
immediately after. You would naturally want to clean after working with
bacteria, etc.
Paula
At 01:41 PM 10/16/2000 -0600, Kozoriz, Deneen HE0 wrote:
>Hello fellow flowers
>
>I have a question to all cytometrists using a BD FACSCalibur.
>
>Our flow cytometry department wants to use our FACSCalibur for clinical
>service (blood, bone marrow, fine needle aspirates and tissue) and research
>projects(anti-viral susceptibility testing).
>Is anyone using one machine for different applications? If you are, how do
>you clean the cytometer between applications?
>Do you find running clinical samples then running research then back to
>clinical samples effect your results.
>Has anyone experience difficulty running bleach through the FACSCalibur for
>more than 30 minutes? Has it effected your FACSComp and patient results.
>
>Thanking you in advance.
>
>Deneen E. Kozoriz
>Provincial Health Laboratory
>Regina, Sask. Canada
Paula Fukushima
Flow Cytometry
LP, NCI, NIH
10 Center Drive MSC-1500
Bethesda, Md. 20892-1500
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