Dear all Jim Houston stated: The optimum excitation of 33342 is with the 351 line of the argon laser. With emmision around 460+/-. When using the 325 line of the HeCd can I expected a lower emmision output? I would like to run the SP population protocol that was published by Stroms et al. in Blood 15 September 2000-Vol 96. They indicated using the 351 line in combination with a 450DF20 filter for the Blue and a LP675 for the Red. The Question??? Will the 450/20 work with the 325 line or do I need expect it to shift down.?? Any help is appreciated. *********************** Reply: Emission from fluorochromes is generally independent of excitation wavelength (as long as its exciting the same electron orbits). Therefore keep the 450DF20 and LP675. However, you will need to adjust the filter which sends laser light to the 90° (wide angle) scatter detector. Regards, Nigel Blackhall School of Biosciences Plant Science Division University of Nottingham Biological Sciences Building University Park Nottingham NG7 2RD UK Tel: +44 (0)115 9515151 ext 18501 Fax: +44 (0)115 9513298 Email: Nigel.Blackhall@Nottingham.ac.uk http://plantsci.nottingham.ac.uk http://www.nottingham.ac.uk
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