chromosomes

From: Julie Auger (jauger@flowcity.bsd.uchicago.edu)
Date: Tue Oct 10 2000 - 17:12:45 EST


Hi -
Has anyone tried looking at chromosomes on a benchtop instrument?  We are
looking at muntjak cells (with only 3 chromosomes) stained with PI.  We
have tried on our new BD LSR but seemed to have significant fluorescence
"noise" when increasing the FSC detector to max.  Is the 15mW laser just
too weak to be able to detect them?

Suggestions?
Julie

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Julie A. Auger
Director, Immunology Applications Core Facility
University of Chicago
5841 S. Maryland Ave. MC-1089
Chicago, IL  60637

e-mail:   jauger@flowcity.bsd.uchicago.edu
telephone:  773-702-9212 lab   773-702-9261 office
fax:  773-834-5251
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