In my humble opinion you are using too much Triton X. You should scale back to 0.1-0.2%. Check the following reference out for a good protocol: ------- Estimation of Nuclear DNA Content of Plants by Flow Cytometry, K. Arumuganathan and E.D. Earle, Plant Mol. Biology Reporter Vol9(3)1991, pg229-233 ------- I have used this protocol on a variety of species with great success with the addition of a polyamine buffer from the following reference: ------- R.J.Bino, J.N.De Vries, H.L.Kraak and J.G. Van Pijlen, Flow cytometric determination of nuclear replication stages in tomato seeds during priming and germination.Annals of Botany 69, 231-236, 1992 ------- At 07:03 PM 9/30/2000 +0200, Jaroslav Dolezel wrote: >> "Raise the salt John!" But that has not seemed to help the situation. So, >> this buffer has .5% Triton X in it. Is that maybe too high? What is a >good - Derek Schulze Flow Cytometry and Confocal Microscopy Core Facility Cancer Research Labs 353 Botterell Hall Queen's University Kingston, Ontario K7L 3N6 Canada http://meds.queensu.ca/medicine/crl/
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