Snezna, These analyzers work as closed systems, from sheath to waste. As a result, sometimes the waste side of the fluidics can be clogged or restricted. This may result in a slowing of flow through the flow cell, thus increasing the time each event spends in the laser beam. The result is a remarkable increase in all signals (do you see this effect on light scatter as well?). Check to be sure that there is no restriction to the waste tank. Other than that, I'm sure there are also electronic problems that can cause this effect . . . go to BD for help there. MAK. Snezna Rogelj wrote: > Part 1.1 Type: Plain Text (text/plain) > Encoding: quoted-printable -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry <http://www.cancer.med.umich.edu/flow_cytometry> phone: 734-647-3216 fax: 734-936-7376 kukuru@umich.edu
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