>From: "Olson, Douglas" <DOLSON4@partners.org> >To: "'Kerstin'" <beckk@sun11.ukl.uni-freiburg.de> >Subject: RE: specific DC-Marker >Date: Mon, 2 Oct 2000 08:24:05 -0400 >MIME-Version: 1.0 >Status: > >Kesrtin: >CD1a is a poor marker to sue - the kinetics of expression upregulate and >downregulate the marker in a very small window of time - so if you miss >it, too >bad. Try CD83, along with CD80 and CD86. For those generated from MO, CD14 >expression will be very high. >Also, HLA-dr is very high on all DCs. >Finally, run your SSC on log - this seperates them very nicely from all the >other cells. >Hope this helps. > >Best, >do > >--------------------------------------- >Douglas P. Olson >Experimental Hematology/AIDS Research Center >Massachusetts General Hospital >Harvard Medical School >149 13th Street >Boston, MA 02129 >(617)724-2668 - Phone >(617)726-4691 - Fax >Dolson4@partners.org - Email > > >> -----Original Message----- >> From: Kerstin [SMTP:beckk@sun11.ukl.uni-freiburg.de] >> Sent: Thursday, September 28, 2000 12:42 PM >> To: Cytometry Mailing List >> Subject: specific DC-Marker >> >> >> Hello everybody, >> does anyone know a specific marker (except CD1a) for Dendritic cells which >> express mature and immature DCs? I want to seperate Monocytes, makrophages >> and DC with FACS. Are there any differences in expression between >> dendritic cells generated of CD34+progenitor cells, DC generated of >> Monocytes or DC of the skin? >> Thanks for your help. >> Kerstin >> >> ***************************************** >> Kerstin Beck >> Institut für Medizinische Mikrobiologie und Hygiene >> Abteilung Virologie >> Hermann-Herder-Strasse 11 >> D-79104 Freiburg >> Telefon : +49-(0)761-203-6646 >> Fax : +49-(0)761-203-6603 >> beckk@ukl.uni-freiburg.de >> ***************************************** >> > ***************************************** Kerstin Beck Institut für Medizinische Mikrobiologie und Hygiene Abteilung Virologie Hermann-Herder-Strasse 11 D-79104 Freiburg Telefon : +49-(0)761-203-6646 Fax : +49-(0)761-203-6603 beckk@ukl.uni-freiburg.de *****************************************
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