Hi Sandeep. Don't know about an *accepted* protocol as quantitation tends to be a bit of a fraught subject but there is an article written by Ricardo Morilla and Mariano Scolnik available in pdf format on the Royal Microscopy Society's website at: http://www.rms.org.uk/cytom.html I think this would be a good place to start. Derek On Fri, 29 Sep 2000, sandeep ramalingam wrote: > How does one go about quantifying CD4 molecules per T > cell. Is there an accepted protocol. Hoping to hear > from you. ************************************************************************ Derek Davies Voice: (44) 020 7269 3394 FACS Laboratory, FAX: (44) 020 7269 3100 Imperial Cancer Research Fund, e_mail: derek.davies@icrf.icnet.uk London, UK mobile: 07790 604112 Web Page: http://www.icnet.uk/axp/facs/davies/index.html In tenebris lux *************************************************************************
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