Hi all, someone wants us to look at basal level of expression of IL-10 on monocytes/lymphocytes. There was a discussion on the group ealier regarding defining a monocyte, and most people suggested the use of markers such as CD33, CD14, CD64 and CD163. The marker has to be BRIGHT because the group of patients is notorious for poor RBC lysis. I tried BD's CD33FITC but it didn't "stick out" enough, I really like how bright the CD14PE is on monos - is that a good idea? Is the CD14PerCP/TC as bright on monos? The less compensation the better :) Maciej ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Yahoo! Photos - 35mm Quality Prints, Now Get 15 Free! http://photos.yahoo.com/
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:33 EST