Annexin in adhesive cells

From: Kenichi Fujise (kenichi.fujise@uth.tmc.edu)
Date: Mon Oct 02 2000 - 10:02:30 EST 

Dear Colleagues:

I would be appreciative if somebody could tell me whether Annexin
experiment using flow cytometry can be performed in adhesive cells.  My
concern was that harvesting adhesive cells using Trypsin-EDTA would
reduce the Annexin signals significantly.

If anybody has tried to gate cells that are successfully transfected
with a certain gene somehow, I would be appreciative of knowing how it
was done.  I wonder if anybody has used GFP to achieve this.

I thank you for your time and help on this.

Sincerely,


Dr. Ken Fujise

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