Dear fellow flowers... I am trying to detect IL-10 intracellularly in melanoma tumor cells and PBL's. I am looking for permeabilization reagents and amplification of signal recommendations. The tenative scenario is-- mouse anti human IL-10 with goat anti mouse-PE. Is there a better way to amplify signal? Also, I do not want to use any golgi-stop reagents to increase the IL-10. Thank you for any recommendations... Lynn Kord _________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com. Share information about yourself, create your own public profile at http://profiles.msn.com.
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