Summary of Flow-FISH responses
From: Bill Telford (telfordw@box-t.nih.gov)
Date: Thu Sep 28 2000 - 13:39:31 EST
Hello all...
Thanks to Vincent Shankey, Lyndsay
Smith, Liusheng He, Tom Just and
everyone else who responded to my query regarding Flow-FISH. Here
is a summary of the responses I received...
The recommended primary references are:
"Telomere length dynamics in human lymphocyte
subpopulations measured by flow cytometry "
N Rufer et al, Nature Biotech (1998) 16, 743-747 .
"Telomere analysis by fluorescence in situ hybridisation and flow
cytometry"
Hultin M et al, Nucleic Acids Res (1998) 26 (16), 3651-3656
.
The subject was recently reviewed in:
"Flow cytometric measurement of telomere length"
Lauzon W et al, Clinical Cytometry (2000) 42, 159-164.
The FITC labelled telomeric probe can be had from PE Biosystems.
The UK contact info is:
PE Biosystems
Kelvin Close
Birchwood service park north
Warrington
WA3 7PB
UK
Fax 01925 282502
Tom Just from Dako also responded that they have a PNA probe for telemore
length estimation as well.
Once again, thanks to everyone!
Bill Telford
NCI Medicine Branch Flow Lab
DCS-NCI-NIH
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: Sat Mar 10 2001 - 19:31:33 EST