Hello everyone,
We have a user who has tried to stain CHO cells with Hoechst 342. She
has tried 10ug/ml Ho342 for various time points (15, 30, 45 and
60minutes) at 37 degrees C. She has also tried using 2.5ug/ml and
5ug/ml verapamil along with the hoechst staining. We are seeing
improvement in staining with increasing time of incubation with Ho342
but the staining is still suboptimal (very broad CV for G1).
Verapamil didn't have much of an effect. Has anyone stained this cell
line with Ho342 and has a special modification they would be willing
to share? Thank you for your help.
Lucy Brown
Beckman Research Institute/City of Hope
626-359-8111 X3306
lbrown@coh.org
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