Dear Flowers: I am interested in sperm testing in FACSCalibur. We use rhodamine 123 and PI to test rat sperm cells. However, all the cells are stained both dyes. The incubation temperature is 37 centigrade degrees. Low flow rate is set to deliver the sample to the flow cell. We would like to seeking help from those expertise at sperm flow analysis. We really appreciate if you can give us some suggestions and comments on what else we need to take into consideration before we analyse sperms in Calibur? Thank you very much. Yen-Chi Chen, M.S. CAD Application Specialist BD Worldwide Inc., Taiwan Branch Taipei, Taiwan, R.O.C.
This archive was generated by hypermail 2b29 : Sat Mar 10 2001 - 19:31:33 EST